4RJI
Acetolactate synthase from Bacillus subtilis bound to ThDP - crystal form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-19 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 140.647, 140.647, 238.877 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.664 - 3.200 |
R-factor | 0.1901 |
Rwork | 0.187 |
R-free | 0.25280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ozf |
RMSD bond length | 0.005 |
RMSD bond angle | 0.888 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.390 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 40142 | |
Completeness [%] | 99.5 | 98.9 |
Redundancy | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30 % (v/v) polyethylene glycol 300, 200 mM Ca acetate, 100 mM Na cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |