4RJ2
Crystal structure of E.coli purine nucleoside phosphorylase at 0.99 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.120, 110.220, 88.210 |
| Unit cell angles | 90.00, 111.08, 90.00 |
Refinement procedure
| Resolution | 16.280 - 0.990 |
| R-factor | 0.17623 |
| Rwork | 0.176 |
| R-free | 0.18866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.432 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.040 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.100 | 0.670 |
| Number of reflections | 718472 | |
| <I/σ(I)> | 2.11 | |
| Completeness [%] | 98.4 | 98.05 |
| Redundancy | 3.71 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | COUNTER DIFFUSION |
