4RIF
Landomycin Glycosyltransferase LanGT2, carbasugar substrate complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 57.970, 59.710, 71.990 |
Unit cell angles | 87.30, 73.97, 64.92 |
Refinement procedure
Resolution | 50.360 - 1.850 |
R-factor | 0.1769 |
Rwork | 0.176 |
R-free | 0.19820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | unliganded structure |
RMSD bond length | 0.010 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.360 |
High resolution limit [Å] | 1.850 |
Rmerge | 0.072 |
Number of reflections | 62872 |
<I/σ(I)> | 7.5 |
Completeness [%] | 87.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.8 | 293 | 1.3 M sodium citrate 0.1 M HEPES/NaOH, pH 6.8, VAPOR DIFFUSION, temperature 293K |