4RHP
Crystal structure of human COQ9 in complex with a phospholipid, Northeast Structural Genomics Consortium Target HR5043
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.340, 97.628, 64.109 |
| Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
| Resolution | 48.790 - 2.393 |
| R-factor | 0.1859 |
| Rwork | 0.179 |
| R-free | 0.24610 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.089 | 0.514 |
| Number of reflections | 35868 | |
| <I/σ(I)> | 15.68 | 2.55 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | mircobatch under oil | 8.5 | 291 | 100 mM TRIS (pH 8.5), 20% (v/v) PEG 3350, and 200 mM MgCl2 , mircobatch under oil, temperature 291K |
