4RHN
HISTIDINE TRIAD NUCLEOTIDE-BINDING PROTEIN (HINT) FROM RABBIT COMPLEXED WITH ADENOSINE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1994-09 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 50.850, 50.850, 81.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.26400 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 25.200 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PROTSYS |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.820 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.030 * |
Total number of observations | 50474 * |
Number of reflections | 9074 |
<I/σ(I)> | 10 |
Completeness [%] | 84.0 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * | 30% POLYETHYLENE GLYCOL 8000, 0.1 M SODIUM ACETATE,N0.1 M SODIUM CACODYLATE, PH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 30 (%) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
5 | 1 | drop | adenosine | 0.2 (mM) | |
6 | 1 | drop | 150 (mM) | ||
7 | 1 | drop | Tris-Cl | 20 (mM) | pH7.4 |