4RHE
Crystal structure of UbiX, an aromatic acid decarboxylase from the Colwellia psychrerythraea 34H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-10 |
Detector | ADSC QUANTUM 270r |
Wavelength(s) | 0.97934 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.204, 141.908, 170.069 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.420 - 2.003 |
R-factor | 0.20409 |
Rwork | 0.202 |
R-free | 0.25024 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 2.010 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.539 | |
High resolution limit [Å] | 2.003 | 2.003 |
Rmerge | 0.120 | |
Number of reflections | 85707 | |
Completeness [%] | 98.3 | 96.37 |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.8 | 293 | 200 mM sodium chloride, 100 mM potassium phosphate monobasic/sodium phosphate dibasic, pH 5.8, 11% w/v PEG8000, EVAPORATION, temperature 293K |