4RHD
DNA Duplex with Novel ZP Base Pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2013-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 3 |
| Unit cell lengths | 59.429, 59.429, 87.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.050 - 1.700 |
| R-factor | 0.22361 |
| Rwork | 0.223 |
| R-free | 0.22649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 3.198 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 87.050 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 37630 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% (v/v) MPD, 40 mM Sodium cacodylate, 12 mM Spermine tetra-HCl, pH 6.0, 80 mM NaCl, 20 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
