4RH8
Crystal structure of the outer membrane lipopolysaccharide transport protein LptE (RlpB) from Escherichia coli in the tetragonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Detector technology | PIXEL |
Collection date | 2011-03-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 102.460, 102.460, 166.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.310 - 2.200 |
R-factor | 0.22717 |
Rwork | 0.225 |
R-free | 0.26176 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nhr |
RMSD bond length | 0.018 |
RMSD bond angle | 1.998 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 1.570 | |
Number of reflections | 45803 | |
Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.8 M sodium formate, 0.1 M Tris/HOAc pH 8.50, 15% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |