4RH1
Spore photoproduct lyase C140A/S76C mutant with bound AdoMet and dinucleoside spore photoproduct
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.07169 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.440, 58.560, 130.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.530 - 2.600 |
R-factor | 0.26693 |
Rwork | 0.264 |
R-free | 0.32490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.999 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.530 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.458 | |
Number of reflections | 13108 | |
Completeness [%] | 99.0 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 200 mM lithium sulfate, 100 mM Tris-HCl, 19-27% PEG800, 70 mM HEGA-8, anaerobic conditions, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |