4RGL

Crystal structure of a Fic family protein (Dde_2494) from Desulfovibrio desulfuricans G20 at 2.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97895,0.97929,0.91837
Spacegroup name	P 43 21 2
Unit cell lengths	56.129, 56.129, 251.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.908 - 2.700
R-factor	0.229
Rwork	0.227
R-free	0.26270
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	0.380
Data reduction software	XDS
Data scaling software	XSCALE (January 10, 2014 BUILT=20140307)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.908	41.910	2.800
High resolution limit [Å]	2.700	5.770	2.700
Rmerge	0.086	0.045	1.261
Number of reflections	11879	1384	1123
<I/σ(I)>	13.33	30.4	1.7
Completeness [%]	99.3	98.8	94.8
Redundancy	6.83		6.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	277	40.0% MPD, 0.1M CAPS pH 10.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K