4RG9
Crystal structure of APC3-APC16 complex (Selenomethionine Derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 |
| Unit cell lengths | 116.019, 116.019, 184.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.940 - 3.250 |
| R-factor | 0.21166 |
| Rwork | 0.210 |
| R-free | 0.24846 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.067 |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.390 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 38356 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 0.1M MES, pH 6.2, 0.24M magnesium chloride, 6% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
