4RG8
Structural and biochemical studies of a moderately thermophilic Exonuclease I from Methylocaldum szegediense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.967, 97.020, 111.411 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.080 - 2.120 |
| R-factor | 0.22433 |
| Rwork | 0.223 |
| R-free | 0.25292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.029 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.080 | 2.170 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 36277 | |
| Completeness [%] | 99.2 | 94.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20% PEG6K, 0.1 M Tris pH8, 0.2 M calcium chloride , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
