4RG8
Structural and biochemical studies of a moderately thermophilic Exonuclease I from Methylocaldum szegediense
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.967, 97.020, 111.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.080 - 2.120 |
R-factor | 0.22433 |
Rwork | 0.223 |
R-free | 0.25292 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fxx |
RMSD bond length | 0.005 |
RMSD bond angle | 1.029 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.080 | 2.170 |
High resolution limit [Å] | 2.120 | 2.120 |
Number of reflections | 36277 | |
Completeness [%] | 99.2 | 94.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20% PEG6K, 0.1 M Tris pH8, 0.2 M calcium chloride , VAPOR DIFFUSION, SITTING DROP, temperature 298K |