4REX
Crystal structure of the first WW domain of human YAP2 isoform
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-24 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 42.673, 43.101, 21.302 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.551 - 1.600 |
R-factor | 0.1785 |
Rwork | 0.178 |
R-free | 0.19420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ho2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.356 |
Data scaling software | Aimless (0.2.13) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.670 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.104 | 0.582 |
Number of reflections | 5514 | |
<I/σ(I)> | 12 | 3.2 |
Completeness [%] | 99.2 | 96.9 |
Redundancy | 6.9 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 293 | 1.5 M ammonium sulphate, 0.1 M sodium acetate, 100 mM proline, 5 mM NDSB-201 and 5mM MBCD, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |