4REV
Structure of the dirigent protein DRR206
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98093 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.416, 88.416, 196.767 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.001 - 1.950 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.037 | 0.661 |
| Number of reflections | 21935 | |
| <I/σ(I)> | 21.9 | 2.6 |
| Completeness [%] | 99.6 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 295 | 4% 1-butyl-2,3-dimethylimidazolium tetrafluoroborate (w/v), 0.1M Bis-Tris propane pH 7.8, and 35% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
