4RDS
Lysozyme crystallized with red food coloring dye
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.890, 78.890, 36.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.280 - 1.230 |
| R-factor | 0.15403 |
| Rwork | 0.153 |
| R-free | 0.17345 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code: 193L |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.260 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.077 | 0.919 |
| Number of reflections | 33363 | |
| <I/σ(I)> | 18.15 | 2.83 |
| Completeness [%] | 97.2 | 99.9 |
| Redundancy | 11.69 | 6.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.2M NaCl, 25% Ethylene Glycol, 50mM NaOAc(pH 4.5), schilling Red Food coloring dye., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
