4RDK
Crystal structure of Norovirus Boxer P domain in complex with Lewis b tetrasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 139.866, 139.866, 64.789 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.295 - 1.629 |
| R-factor | 0.133 |
| Rwork | 0.131 |
| R-free | 0.16650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rdj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.054 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.629 | 1.629 |
| Rmerge | 0.079 | 0.503 |
| Number of reflections | 90264 | |
| <I/σ(I)> | 43.2 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M LiCl, 18%(w/v) PEG 3350, 10%(v/v) MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
