4RDB

Crystal structure of an immunoreactive 32 kDa antigen PG49 (PG_0181) from Porphyromonas gingivalis W83 at 1.45 A resolution (PSI Community Target, Nakayama)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184,0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	39.636, 86.821, 90.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.411 - 1.450
R-factor	0.1191
Rwork	0.117
R-free	0.15690
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.605
Data reduction software	XDS
Data scaling software	XSCALE (January 10, 2014 BUILT=20140307)
Phasing software	SHELX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.411		1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.083	0.027	0.714
Number of reflections	55908	5928	5264
<I/σ(I)>	13.38	39.2	2.3
Completeness [%]	99.1	98.7	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.17M sodium acetate, 15.0% Glycerol, 28.5% polyethylene glycol 4000, 4.0% Acetone, 0.1M TRIS pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K