4RD7
The crystal structure of a Cupin 2 conserved barrel domain protein from Salinispora arenicola CNS-205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 43.687, 43.687, 132.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.497 - 1.571 |
| R-factor | 0.1574 |
| Rwork | 0.155 |
| R-free | 0.19380 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.105 |
| Data reduction software | DENZO (Builder/HKL3000) |
| Data scaling software | SCALEPACK (Builder/HKL3000) |
| Phasing software | SHELXD (Builder/HKL3000) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.124 | 0.572 |
| Number of reflections | 18841 | |
| <I/σ(I)> | 47.2 | 3.9 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 14 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 1.6M Ammonium Sulfate, 0.1M MES:NaOH, 10% (v/v) Dioxane, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
