4RCX

Trichomonas vaginalis triosephosphate isomerase TVAG_497370 gene (Ile-45 variant) loop 3 deletion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-15
Detector	RAYONIX MX-300
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	46.970, 56.730, 105.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.960 - 1.750
R-factor	0.1747
Rwork	0.173
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.626
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1730))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.963
High resolution limit [Å]	1.750
Rmerge	0.065
Number of reflections	29120
<I/σ(I)>	11.2
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.2 M Calcium acetate, 0.1 M Sodium cacodylate, 18 % PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K