4RCL
Structure of EspG3 chaperone from the type VII (ESX-3) secretion system, space group P43212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 92.679, 92.679, 158.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.340 - 2.700 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l4w |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.340 | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 36408 | |
| <I/σ(I)> | 19.8 | 2.3 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 11.1 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 295 | 0.1M SODIUM CACODYLATE, PH 6.0, 15% PEG200, 5% PEG3350, vapor diffusion, hanging drop, temperature 295K |
