4RCK
Crystal Structure of Uncharacterized Membrane Spanning Protein from Vibrio fischeri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 65 |
Unit cell lengths | 49.734, 49.734, 343.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.508 - 2.999 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23700 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.845 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_1745)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 9679 | |
<I/σ(I)> | 9.8 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.5 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Magnesium formate, 20 %(w/v) PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |