4RCI
Crystal structure of YTHDF1 YTH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979237 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.519, 82.136, 123.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.970 |
R-factor | 0.1903 |
Rwork | 0.189 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3i |
RMSD bond length | 0.013 |
RMSD bond angle | 1.306 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.17) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.660 | 48.660 | 2.020 |
High resolution limit [Å] | 1.970 | 9.030 | 1.970 |
Rmerge | 0.088 | 0.025 | 0.967 |
Total number of observations | 4209 | 29482 | |
Number of reflections | 57668 | ||
<I/σ(I)> | 17.2 | 57 | 2.3 |
Completeness [%] | 99.9 | 98.5 | 99.7 |
Redundancy | 7.3 | 6.1 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 25% PEG-3350, 0.2 M potassium thiocyanate, vapor diffusion, temperature 293K |