4RC7
Crystal structure of cyanobacterial aldehyde-deformylating oxygenase F86YF87Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.7382 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.014, 62.109, 125.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.407 - 2.200 |
| R-factor | 0.20676 |
| Rwork | 0.205 |
| R-free | 0.23763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4quw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 24713 | |
| Completeness [%] | 98.5 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 291 | 0.02M adenosine-5'-triphosphate disodium salt hydrate, 0.2M L-proline, 0.1M Hepes pH 7.1, 25%(w/v) PEG1500 , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
