4RC5
Crystal structure of cyanobacterial aldehyde-deformylating oxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.656, 61.833, 124.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.944 - 2.300 |
| R-factor | 0.21024 |
| Rwork | 0.209 |
| R-free | 0.23789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4quw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21848 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 291 | 0.2M L-proline, 0.1M Hepes, 25%(w/v) PEG1500, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
