4RBV
PduA K26A S40GSG mutant, from Salmonella enterica serovar Typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 236.800, 236.800, 236.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.531 - 3.100 |
| R-factor | 0.1967 |
| Rwork | 0.194 |
| R-free | 0.21830 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 |
| Data scaling software | XSCALE |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.531 | 3.180 | |
| High resolution limit [Å] | 3.100 | 13.870 | 3.100 |
| Rmerge | 0.076 | 0.032 | 0.694 |
| Number of reflections | 19474 | 143 | 1439 |
| <I/σ(I)> | 11.61 | 30.18 | 1.73 |
| Completeness [%] | 97.4 | 57 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Potassium sulfate, 2.2M Ammonium sulfate, vapor diffusion, hanging drop, temperature 298K |
