4RBQ
32 base pair oligo(U) RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97946 |
Spacegroup name | H 3 2 |
Unit cell lengths | 42.890, 42.890, 266.936 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.790 - 1.050 |
R-factor | 0.1312 |
Rwork | 0.131 |
R-free | 0.15370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IDEAL A-FORM RNA |
RMSD bond length | 0.004 |
RMSD bond angle | 0.898 |
Data reduction software | MOSFLM |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.3_927)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.800 | |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.057 | |
Number of reflections | 45101 | |
<I/σ(I)> | 20.1 | |
Completeness [%] | 100.0 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 50 mM sodium cacodylate, pH 6.5, 200 mM potassium chloride, 75 mM magnesium acetate, 900 mM lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |