4RAX

A regulatory domain of an ion channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 3
Unit cell lengths	89.492, 89.492, 89.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.535 - 1.450
R-factor	0.1525
Rwork	0.151
R-free	0.17590
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.065
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.079
Number of reflections	42493
Completeness [%]	99.9	99.7	100
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2M Magnesium chloride, 0.1M Hepes pH 7.5, 25% w/w PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K