4RAV
Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.07169 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.313, 35.933, 110.946 |
Unit cell angles | 90.00, 120.72, 90.00 |
Refinement procedure
Resolution | 44.236 - 2.500 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.718 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.690 | 47.690 | 2.640 |
High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
Rmerge | 0.067 | ||
Number of reflections | 18107 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 99.1 | 98.4 | 97.8 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.2 M ammonium sulphate, 25% PEG 4000 and 0.1 M sodium acetate buffer at pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |