4RA9
Crystal Structure of Conjoint Pyrococcus Furiosus L-asparaginase with Citrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-11-14 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 90.679, 90.679, 189.955 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.370 - 2.049 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nje |
RMSD bond length | 0.008 |
RMSD bond angle | 1.067 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.049 | 2.050 |
Rmerge | 0.089 | 0.790 |
Number of reflections | 29876 | |
<I/σ(I)> | 38.8 | 2.05 |
Completeness [%] | 99.8 | 97.5 |
Redundancy | 14.5 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 200MM SODIUM CITRATE TRIBASIC DIHYDRATE, 100MM SODIUM CACODYLATE, 30%(V/V) 2-PROPANOL, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |