4RA2
PP2Ca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2014-03-30 |
| Detector | SYNTEX |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 90.714, 90.714, 105.443 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.810 - 1.940 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a6q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Number of reflections | 35772 | |
| Completeness [%] | 95.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 277.15 | 15(v/v)% PEG8000, pH 5.5, 50mM K2HPO4, 15% 2mMDTT, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
