4R9Z
Mycobacterium avium subs paratuberculosis tesB protein MAP1729c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.230, 70.110, 137.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.055 - 2.600 |
R-factor | 0.2047 |
Rwork | 0.202 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rd7 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.080 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.060 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | 0.429 |
Number of reflections | 17735 | |
<I/σ(I)> | 5.9 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 70 mM sodium cacodylate, 1.2 sodium acetate trihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |