4R9X
Crystal Structure of Putative Copper Homeostasis Protein CutC from Bacillus anthracis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-16 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97905 |
Spacegroup name | P 31 |
Unit cell lengths | 58.335, 58.335, 118.967 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.329 - 1.851 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.20600 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.218 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_1745)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 38473 | |
<I/σ(I)> | 24.7 | 2.31 |
Completeness [%] | 99.5 | 100 |
Redundancy | 5.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M Calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40 %(w/v) PEG300, VAPOR DIFFUSION, SITTING DROP, temperature 289K |