4R9V
Crystal structure of sialyltransferase from photobacterium damselae, residues 113-497 corresponding to the gt-b domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97607 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.444, 56.523, 87.375 |
Unit cell angles | 90.00, 119.95, 90.00 |
Refinement procedure
Resolution | 41.350 - 2.300 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z4t |
RMSD bond length | 0.008 |
RMSD bond angle | 1.075 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.067 | 0.320 |
Number of reflections | 17873 | |
<I/σ(I)> | 16.9 | 3.6 |
Completeness [%] | 99.1 | 99.2 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 294 | 20% (w/v) PEG-1000, 100mM imidazole, pH 8.0, 200mM Calcium Acetate, VAPOR DIFFUSION, temperature 294K |