4R9P
An Expansion to the Smad MH2-family: The structure of the N-MH2 expanded domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.840, 45.670, 54.190 |
| Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
| Resolution | 31.267 - 1.592 |
| R-factor | 0.1783 |
| Rwork | 0.176 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1701)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.270 |
| High resolution limit [Å] | 1.590 |
| Rmerge | 0.069 |
| Number of reflections | 23152 |
| <I/σ(I)> | 13.58 |
| Completeness [%] | 95.5 |
| Redundancy | 5.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 12% v/v 1,2-Propanediol, 9% w/v PEG 20000, 0.1M Glycine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
