4R9J

L-ficolin complexed to glucosamine-6-sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 32 2 1
Unit cell lengths	97.170, 97.170, 139.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 2.100
R-factor	0.17394
Rwork	0.172
R-free	0.20336
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.947
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.150
High resolution limit [Å]	2.100	9.400	2.100
Number of reflections	45165
Completeness [%]	99.8	87.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	Protien solution: 5.7 mg/ml in 145 mM NaCl, 50 mM triethanolamine-HCl, pH 7.4. Reservoir solution composed of 15% (w/v) PEG 8000, 200 mM Ca acetate and 0.1 M HEPES. Cryoprotection was achieved by adding PEG 400 to the drop just before flash-cooling the crystal in liquid nitrogen, EVAPORATION, temperature 293K