4R9H
Crystal structure of dimeric S33C beta-2 microglobulin mutant at 1.9 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 68.840, 68.840, 200.039 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.840 - 1.900 |
| R-factor | 0.21353 |
| Rwork | 0.212 |
| R-free | 0.24736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ov6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.402 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.840 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.109 | 0.940 |
| Number of reflections | 38985 | |
| <I/σ(I)> | 12.4 | 2.2 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 8.9 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG4000, 0.2 M imidazole-malate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
