4R8J
d(TCGGCGCCGA) with lambda-[Ru(TAP)2(dppz)]2+ soaked in D2O
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-14 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.370, 42.370, 39.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.660 - 1.210 |
R-factor | 0.11 |
Rwork | 0.109 |
R-free | 0.12500 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 2.592 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 18.660 |
High resolution limit [Å] | 1.210 |
Number of reflections | 11471 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 291 | The drops contained 1 l of 2mM oligonuclotide, 1 l of 4mM rac [Ru(TAP)2(dppz)]Cl2 and 6 l of the following solution; 10% (vol/vol) MPD, 40 mM sodium cacodylate (pH 6.0), 12mM spermine tetra-HCl, 80mM sodium chloride and 20 mM barium chloride. The drop was equilibrated against 1ml of 35% (vol/vol) 2-methyl-2,4-pentanediol (MPD) in H2O , VAPOR DIFFUSION, SITTING DROP, temperature 291K |