4R86
Crystal Structure of Aminoglycoside/Multidrug Efflux System AcrD from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97931 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 91.167, 91.167, 147.402 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.584 - 3.001 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.22400 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.784 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_1745)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.070 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 14557 | |
<I/σ(I)> | 10.6 | 2.4 |
Completeness [%] | 98.9 | 99.8 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 47.1 % w/v PEG 1000, 150 mM Tris pH 8.0, 30 mM Potassium Bromide, VAPOR DIFFUSION, SITTING DROP, temperature 289K |