4R7E

Structure of Bre1 RING domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	200
Detector technology	PIXEL
Collection date	2013-06-28
Detector	PSI PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	P 61 2 2
Unit cell lengths	56.765, 56.765, 134.858
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.160 - 2.251
R-factor	0.2231
Rwork	0.221
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.236
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	26.160
High resolution limit [Å]	2.250
Number of reflections	11396
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	2.0 M ammonium sulfate and 0.1 mM sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K