4R73

Structure of the periplasmic binding protein AfuA from Actinobacillus pleuropneumoniae (endogenous glucose-6-phosphate and mannose-6-phosphate bound)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Detector	RAYONIX MX-300
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	43.530, 95.320, 152.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.596 - 1.600
R-factor	0.1623
Rwork	0.161
R-free	0.19420
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.641
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.596	39.500	1.670
High resolution limit [Å]	1.600	6.660	1.600
Number of reflections	83626
Completeness [%]		98.2	88.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.2 M magnesium chloride, 0.1 M Tris, pH 6.5, 25% PEG3350, 20% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K