4R71
Structure of the Qbeta holoenzyme complex in the P1211 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-06 |
| Detector | PILATUS 2M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.730, 115.450, 178.520 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 59.827 - 3.210 |
| R-factor | 0.2158 |
| Rwork | 0.213 |
| R-free | 0.26760 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.546 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.320 | 3.290 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.594 | |
| Number of reflections | 65129 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 20% PEG 4000, 5% PEG 400, 0.4M ammonium sulfate, 0.1M sodium acetate, 0.01M betaine hydrochloride, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
