4R70

Crystal structure of bacteriophytochrome RpBphP3 from photosynthetic bacterium R. palustris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-17
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 31 2 1
Unit cell lengths	143.307, 143.307, 120.512
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.998 - 2.850
R-factor	0.1891
Rwork	0.185
R-free	0.25960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	2.468
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Rmerge	0.073
Number of reflections	33613
<I/σ(I)>	52
Completeness [%]	98.2
Redundancy	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	10mg/ml protein, 0.1M magnesium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K