4R6Y
Crystal structure of solute-binding protein stm0429 from salmonella enterica subsp. enterica serovar typhimurium str. lt2, target efi-510776, a closed conformation, in complex with glycerol and acetate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-18 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.185, 67.601, 77.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.220 |
R-factor | 0.11469 |
Rwork | 0.114 |
R-free | 0.14781 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.914 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.240 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.062 | 0.500 |
Number of reflections | 85094 | |
<I/σ(I)> | 25.9 | 2.1 |
Completeness [%] | 98.0 | 77.4 |
Redundancy | 6.7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | PROTEIN: 10 MM BIS-TRIS, 500 MM NACL, 5% GLYCEROL, 5 MM DTT, TEV PROTEASE (1:100); RESERVOIR: 0.17 M AMMONIUM ACETATE, 0.085 M SODIUM CITRATE:HCL, PH 5.6, 25% PEG4000, 15% GLYCEROL; VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |