4R6K
Crystal structure of ABC transporter substrate-binding protein YesO from Bacillus subtilis, TARGET EFI-510761, an open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-26 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.932, 75.727, 119.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.940 |
| R-factor | 0.19383 |
| Rwork | 0.192 |
| R-free | 0.25981 |
| Structure solution method | MR |
| Starting model (for MR) | 2dvu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.079 | 0.780 |
| Number of reflections | 30836 | |
| <I/σ(I)> | 25 | 1.7 |
| Completeness [%] | 99.3 | 94.2 |
| Redundancy | 6.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | Protein: 10 mM BIS-TRIS, 500 mm NaCl, 5% glycerol, 5 mm DTT; reservoir: 1.4 m sodium citrate, 0.1 m HEPES-NaOH, pH 7.5; vapor diffusion, sitting drop, temperature 294K |
