4R6C
X-ray diffraction in temporally and spatially resolved biomolecular science: the X-ray crystal structure of hen egg white lysozyme cocrystallized with Ta6Br12 and then a crystal soaked in K2PtBr6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.070, 79.070, 37.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.360 - 1.700 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.23046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.229 |
| Data reduction software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.020 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.049 |
| Number of reflections | 24843 |
| Completeness [%] | 100.0 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 4.5 | 293 | NaCl; Ta6Br12 2mM. A crystal was soaked in K2PtBr6 10mM., pH 4.5, Batch, temperature 293K |
