4R6A
Crystal structure of human TLR8 in complex with Hybrid-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-06-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.143, 122.211, 107.778 |
| Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
| Resolution | 26.090 - 2.100 |
| R-factor | 0.20375 |
| Rwork | 0.201 |
| R-free | 0.25932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.650 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 89651 |
| <I/σ(I)> | 17 |
| Completeness [%] | 87.1 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.9 | 293 | 10%(w/v) PEG3350, 0.1M sodium citrate pH 4.9, 0.3M potassium formate, temperature 293K |
