4R63
Binary complex crystal structure of R258A mutant of DNA polymerase Beta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-26 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5408 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.300, 79.320, 54.780 |
Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
Resolution | 20.609 - 1.850 |
R-factor | 0.185 |
Rwork | 0.180 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3isb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.137 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.099 | 0.078 | 0.484 |
Number of reflections | 38158 | ||
<I/σ(I)> | 13.8 | ||
Completeness [%] | 99.7 | 98.9 | 99 |
Redundancy | 6.1 | 7.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 50 mM Imidazole, 350 mM Sodium Acetate, 16-18 % PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 291K |