4R60
Crystal Structure of Xaa-Pro dipeptidase from Xanthomonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.324, 105.515, 111.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.462 - 1.830 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fkc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.046 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.074 | 0.631 |
| Number of reflections | 88109 | |
| <I/σ(I)> | 18.1 | 2.1 |
| Redundancy | 7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under-oil | 5.5 | 294 | 40mM KH2PO4, 15% glycerol, 12% PEG8000, pH 5.5, Microbatch under-oil, temperature 294K |
