4R4V
Crystal structure of the VS ribozyme - G638A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.07812 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.229, 102.229, 215.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.072 - 3.070 |
R-factor | 0.1802 |
Rwork | 0.179 |
R-free | 0.20400 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.827 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.130 |
High resolution limit [Å] | 3.070 | 3.070 |
Number of reflections | 21788 | |
Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M bis-Tris pH 6.5 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |