4R4N
Crystal structure of the anti-hiv-1 antibody 2.2c in complex with hiv-1 93ug037 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 112.967, 144.178, 158.351 |
| Unit cell angles | 110.58, 92.30, 99.19 |
Refinement procedure
| Resolution | 49.145 - 3.560 |
| R-factor | 0.2975 |
| Rwork | 0.296 |
| R-free | 0.32810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.985 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.070 |
| High resolution limit [Å] | 3.560 | 3.780 |
| Rmerge | 0.276 | |
| Number of reflections | 71903 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 70.2 | 52.3 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 14% PEG 3350, 1M sodium formate, 0.1M calcium chloride, and 100 mM sodium acetate pH 4.5 , VAPOR DIFFUSION, HANGING DROP, temperature 291 K |
